Monitoring transplanted stem cells in rat Achilles tendon by in vivobioluminescent imaging

doi:10.3969/j.issn.2095-4344.2013.23.009

Abstract

Abstract:

BACKGROUND: The mechanisms for the homing, migration, proliferation and differentiation of transplanted adipose tissue derived stem cells remain unclear. The in vivo bioluminescent imaging system is a newly developed technique for directly detecting the biological behaviors of transplanted cells in vivo.
OBJECTIVE: To demonstrate the feasibility of using in vivo bioluminescent imaging system to monitor the genetically modified adipose tissue derived stem cells transplanted in Achilles tendon of rats.
METHODS: Adipose tissue derived stem cells isolated from the abdominal cavity of Sprague-Dawley rat were transduced with an adenovirus containing the luciferase reporter gene (3×10¹⁰/L), to observe the influence of transfection on the adipose tissue derived stem cells. Subsequently, the transfected cells were implanted into Achilles tendon defects in rats. The in vivo bioluminescent imaging system was used at days 1, 4, 7 and 14 following transplantation to assess the luciferase expression. The cryosections of repaired Achilles tendon of rats were observed under fluorescence microscope at day 28 postoperatively.
RESULTS AND CONCLUSION: No influence on the morphology and proliferation of adipose tissue derived stem cells was observed after transducing in vitro (P > 0.05). On the repaired Achilles tendon, the luciferase gene expression detected with in vivo bioluminescent imaging system at days 1, 4, 7 and 14 was respectively (1.22±0.43)×10⁶, (1.81±0.76)×10⁶, (1.88±0.69)×10⁶ and (0.89±0.26)×10⁵ counts/s (n=6). Abundant adipose tissue derived stem cells with luciferase expression were also seen in tendon cryosections of this side under fluorescence microscope at day 28. The luciferase gene expression was not detected in the control side. Experimental findings demonstrate that the in vivo bioluminescent imaging system can successfully monitor the fluorogene modified adipose tissue derived stem cells that are implanted into the rat Achilles tendon, and adipose tissue derived stem cells are a potential seed cells in tendon tissue engineering.

Key words: stem cells, stem cell transplantation, deficient adenovirus, adipose tissue derived stem cells, adenovirus, cell transfection, luciferase, tendon, bioluminesent imaging, fluorescence expression, tissue engineering, seed cells, provincial grants-supported paper, stem cells photographs-containing paper

CLC Number:

R394.2

Huang De-qing, Gary Balian. Monitoring transplanted stem cells in rat Achilles tendon by in vivobioluminescent imaging[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(23): 4240-4247.

Figures/Tables 3

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